Composite

Part:BBa_K2802003

Designed by: Shamlan M. S. Reshamwala   Group: iGEM18_ICT-Mumbai   (2018-10-05)


P2 ogr - PO promoter - P2 ogr - RBS + RFP + d.Ter

This part is a composite part composed of BBa_K274371, BBa_I746350 and BBa_K518012. A promoter can be cloned upstream of this part, and the P2 ogr phage activator that will be produced will bind to the PO phage promoter to drive expression of the P2 ogr phage activator, forming a genetic amplifier. RFP, which will be produced from the gene cloned downstream of the second ogr gene, will act as a detectable output of this genetic amplifier.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1301
    Illegal AgeI site found at 1413
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None